HLA-A*03:01 (Monomer) protein (no Peptide)
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- HLA-A*03:01
- Protein Type
- Recombinant
- Protein Characteristics
- Monomer
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- no Peptide
- Purpose
- Human HLA-A*03:01 & B2M Monomer Protein
- Sequence
- Gly25-Thr305 (HLA-A*03:01) & Ile21-Met119 (B2M)
- Specificity
- Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M)
- Characteristics
- Recombinant Human HLA-A*03:01 & B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01) and Ile21-Met119(B2M).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Application Notes
- Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening
- Comment
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The HLA-A*03:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HLA-A*03:01
- Molecular Weight
- 48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_002107
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