Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HLA-A*03:01 (Monomer) protein (Biotin,no Peptide)

Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7504384
  • Target
    HLA-A*03:01
    Protein Type
    Recombinant
    Protein Characteristics
    Monomer
    Origin
    Human
    Source
    • 10
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    Biotin,no Peptide
    Purpose
    Biotinylated Human HLA-A*03:01 & B2M Monomer Protein
    Sequence
    Gly25-Thr305 (HLA-A*03:01) & Ile21-Met119 (B2M)
    Specificity
    Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M)
    Characteristics
    Recombinant Biotinylated Human HLA-A*03:01 & B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01) and Ile21-Met119(B2M).
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
  • Application Notes
    Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening
    Comment

    The HLA-A*03:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
    Storage
    -80 °C
    Storage Comment
    Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    HLA-A*03:01
    Molecular Weight
    48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
    NCBI Accession
    NP_002107
You are here:
Support