HLA-A*02:01 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,PRAME)

Catalog No. ABIN7504364

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for HLA-A*02:01 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,PRAME) (ABIN7504364)

Target: HLA-A*02:01

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-305, Monomer

Purification tag / Conjugate: Biotin,His-Avi Tag,PRAME

Purpose: Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

Sequence: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M)and SLLQHLIGL peptide

Specificity: Uni-Prot: A0A140T913 (HLA-A*02:01), P61769 (B2M), SLLQHLIGL

Characteristics: Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLA-A*02:01

Background: PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

Molecular Weight: 50.40 kDa. Due to glycosylation, the protein migrates to 51-58 kDa based on Tris-Bis PAGE result.

UniProt: A0A140T913