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HLA-A*02:01 (Monomer) protein (Biotin,MAGE-A3)

Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7504362
  • Target
    HLA-A*02:01
    Protein Type
    Recombinant
    Protein Characteristics
    Monomer
    Origin
    • 70
    • 4
    Human
    Source
    • 65
    • 4
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    Biotin,MAGE-A3
    Purpose
    Biotinylated Human HLA-A*02:01 & B2M & MAGE-A3 (KVAELVHFL) Monomer Protein
    Sequence
    Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide
    Specificity
    Uni-Prot: A0A140T913 (HLA-A*02:01), P61769 (B2M), KVAELVHFL
    Characteristics
    Recombinant Biotinylated Human HLA-A*02:01 & B2M & MAGE-A3 (KVAELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    HLA-A*02:01
    Background
    Melanoma antigen family A, 3 (MAGE-A3) is a cancer-testis antigen whose expression has been demonstrated in a wide array of malignancies including melanoma, brain, breast, lung and ovarian cancer. In addition, its ability to elicit spontaneous humoral and cellular immune responses has been shown in cancer patients.
    Molecular Weight
    50.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
    UniProt
    A0A140T913
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