MHC, Class I Protein (Monomer) (Ovalbumin)
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target See all MHC, Class I products
- MHC, Class I
- Protein Type
- Recombinant
- Protein Characteristics
- Monomer
- Origin
- Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MHC, Class I protein is labelled with Ovalbumin.
- Purpose
- Mouse H-2K (b) & B2M & OVA (SIINFEKL) Monomer Protein
- Sequence
- His24-Pro297, Ile21-Met119 (B2M) and SIINFEKL peptide
- Specificity
- Uni-Prot: P01901 (H-2K (B)), P01887 (B2M), SIINFEKL
- Characteristics
- Recombinant Mouse H-2K(b) & B2M & OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297, Ile21-Met119(B2M) and SIINFEKL peptide.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MHC, Class I
- Alternative Name
- H-2K (MHC, Class I Products)
- Synonyms
- H-2K Protein, H-2K(d) Protein, H2-K Protein, K-f Protein, Y-FA Protein, YFV Protein, histocompatibility 2, K1, K region Protein, MHC class I antigen YF5 Protein, MHC class I antigen Protein, major histocompatibility complex, class I Protein, H2-K1 Protein, YF5 Protein, OVAR Protein, LOC512672 Protein
- Background
- Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
- Molecular Weight
- 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P01901
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