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- Target See all FFAR1 Proteins
- FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human FFAR1 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))
- Alternative Name
- FFAR1 (FFAR1 Products)
- Synonyms
- FFAR1 Protein, GPR40 Protein, FFA1R Protein, GPCR40 Protein, Gpr40 Protein, free fatty acid receptor 1 Protein, FFAR1 Protein, Ffar1 Protein
- Background
- A member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.
- Molecular Weight
- The human full length FFAR1 protein has a MW of 31.5 kDa
- UniProt
- O14842
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis
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