G Protein-Coupled Receptor 133 Protein (GPR133)
GPR133
Origin: Human
Host: HEK-293 Cells
Synthetic Nanodisc
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- Target See all G Protein-Coupled Receptor 133 (GPR133) Proteins
- G Protein-Coupled Receptor 133 (GPR133)
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human ADGRD1 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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G Protein-Coupled Receptor 133 (GPR133) (AA 26-570) protein (His tag)
GPR133 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- G Protein-Coupled Receptor 133 (GPR133)
- Alternative Name
- ADGRD1 (GPR133 Products)
- Synonyms
- GPR133 Protein, zgc:103546 Protein, PGR25 Protein, E230012M21Rik Protein, adhesion G protein-coupled receptor D1 Protein, ADGRD1 Protein, adgrd1 Protein, Adgrd1 Protein
- Background
- The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
- Molecular Weight
- The human full length ADGRD1 protein has a MW of 96.5 kDa
- UniProt
- Q6QNK2
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