CLPTM1 Protein

Catalog No. ABIN7491611

Product Details

Target: CLPTM1 (Cleft Lip and Palate Associated Transmembrane Protein 1 (CLPTM1))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purpose: Human CLPTM1 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CLPTM1 (Cleft Lip and Palate Associated Transmembrane Protein 1 (CLPTM1))

Alternative Name: CLPTM1 (CLPTM1 Products)

Synonyms: CLPTM1 Protein, cb102 Protein, sb:cb102 Protein, zgc:100803 Protein, HS9 Protein, N14 Protein, CLPTM1, transmembrane protein Protein, cleft lip and palate associated transmembrane protein 1 Protein, CLPTM1 Protein, clptm1 Protein, Bm1_06285 Protein, PTRG_03625 Protein, Clptm1 Protein

Background: Involved in GABAergic but not glutamatergic transmission. Binds and traps GABAA receptors in the endoplasmic reticulum (ER). Modulates postsynaptic GABAergic transmission, and therefore inhibitory neurotransmission, by reducing the plasma membrane expression of these receptors. Altered GABAergic signaling is one among many causes of cleft palate. Might function as a lipid scramblase, translocating lipids in membranes from one leaflet to the other one.

Molecular Weight: The human full length CLPTM1 protein has a MW of 76.1 kDa

UniProt: O96005