GPR65 Protein

Catalog No. ABIN7491665

Product Details

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purpose: Human GPR65 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Alternative Name: GPR65 (GPR65 Products)

Synonyms: tdag8 Protein, htdag8 Protein, TDAG8 Protein, hTDAG8 Protein, Dig1 Protein, Gpcr25 Protein, Tdag8 Protein, G protein-coupled receptor 65 Protein, G-protein coupled receptor 65 Protein, GPR65 Protein, gpr65 Protein, Gpr65 Protein

Background: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421). Plays a role in immune response by maintaining lysosome function and supporting phagocytosis-mediated intracellular bacteria clearance (PubMed:27287411). May have a role in activation-induced cell death or differentiation of T-cells (By similarity).[UniProtKB/Swiss-Prot Function]

Molecular Weight: The human full length GPR65 protein has a MW of 39.3 kDa

UniProt: Q8IYL9

Pathways: cAMP Metabolic Process