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- Target See all HCRTR1 Proteins
- HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human HCRTR1 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 1-46) protein (Fc Tag)
HCRTR1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 1-46), (AA 244-297) protein (His-IF2DI Tag)HCRTR1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 1-425) protein (GST tag)HCRTR1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))
- Alternative Name
- HCRTR1 (HCRTR1 Products)
- Synonyms
- OX1R Protein, Ox1r Protein, Hctr1 Protein, HCRTR1 Protein, hypocretin receptor 1 Protein, hypocretin (orexin) receptor 1 Protein, HCRTR1 Protein, Hcrtr1 Protein
- Background
- The protein is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B.
- Molecular Weight
- The human full length HCRTR1 protein has a MW of 47.5 kDa
- UniProt
- O43613
- Pathways
- Feeding Behaviour
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