GNRHR Protein

Catalog No. ABIN7491657

Product Details

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purpose: Human GNRHR full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Alternative Name: GNRHR (GNRHR Products)

Synonyms: GNRHR1 Protein, GRHR Protein, HH7 Protein, LHRHR Protein, LRHR Protein, GNRHR Protein, GH1 Protein, Lhrhr Protein, GNRHR1/III Protein, GNRHR2 Protein, xgnrhr Protein, gnrhr3/ii Protein, gnrh-r Protein, gonadotropin releasing hormone receptor Protein, gonadotropin-releasing hormone receptor Protein, GNRHR Protein, Gnrhr Protein, gnrhr Protein, gnrh-r Protein

Background: This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008]

Molecular Weight: The human full length GNRHR protein has a MW of 37.6 kDa

UniProt: P30968