C5AR1 Protein
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- Target See all C5AR1 Proteins
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human C5AR1 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
- Alternative Name
- C5AR1 (C5AR1 Products)
- Synonyms
- C5A Protein, C5AR Protein, C5R1 Protein, CD88 Protein, C5aR Protein, C5r1 Protein, Cd88 Protein, D7Msu1 Protein, GPR77 Protein, complement C5a receptor 1 Protein, complement component 5a receptor 1 Protein, C5AR1 Protein, C5ar1 Protein, c5ar1 Protein
- Background
- Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production
- Molecular Weight
- The human full length C5AR1 protein has a MW of 39.3 kDa
- UniProt
- P21730
- Pathways
- Complement System
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