Claudin 2 Protein (CLDN2)

Catalog No. ABIN7491596

Product Details

Target: Claudin 2 (CLDN2)

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purpose: Human CLDN2 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Claudin 2 (CLDN2)

Alternative Name: CLDN2 (CLDN2 Products)

Synonyms: CLDN2 Protein, zgc:92260 Protein, AL022813 Protein, RGD1560247 Protein, claudin 2 Protein, CLDN2 Protein, cldn2 Protein, Cldn2 Protein

Background: This protein belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.

Molecular Weight: The human full length CLDN2 protein has a MW of 24.5 kDa

UniProt: P57739

Pathways: Hepatitis C