Claudin 5 Protein (CLDN5)

Catalog No. ABIN7491602

Product Details

Target: Claudin 5 (CLDN5)

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purpose: Human CLDN5 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Claudin 5 (CLDN5)

Alternative Name: CLDN5 (CLDN5 Products)

Synonyms: AWAL Protein, BEC1 Protein, CPETRL1 Protein, TMVCF Protein, cld5 Protein, awal Protein, bec1 Protein, claudin-5 Protein, cpetrl1 Protein, tmvcf Protein, AI854493 Protein, MBEC1 Protein, Tmvcf Protein, cldn5 Protein, zgc:85723 Protein, zgc:103419 Protein, claudin 5 Protein, Claudin-5 Protein, claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) Protein, claudin 5a Protein, claudin 5b Protein, claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) L homeolog Protein, CLDN5 Protein, cld5 Protein, cldn5 Protein, Cldn5 Protein, cldn5a Protein, cldn5b Protein, cldn5.L Protein

Background: This protein is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome.

Molecular Weight: The human full length CLDN5 protein has a MW of 23.1 kDa

UniProt: O00501

Pathways: Hepatitis C