Claudin 3 Protein (CLDN3)
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- Target See all Claudin 3 (CLDN3) Proteins
- Claudin 3 (CLDN3)
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human CLDN3 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Claudin 3 (CLDN3) (AA 30-80) protein (His-IF2DI Tag)
CLDN3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Claudin 3 (CLDN3) (AA 144-156) protein (mFc Tag)CLDN3 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Claudin 3 (CLDN3) (AA 144-156) protein (Fc Tag)CLDN3 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Claudin 3 (CLDN3) (AA 1-220) protein (GST tag)CLDN3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Claudin 3 (CLDN3) (AA 30-80) protein (Fc Tag)CLDN3 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- Claudin 3 (CLDN3)
- Alternative Name
- CLDN3 (CLDN3 Products)
- Synonyms
- claudin-3 Protein, cpe-r2 Protein, cpetr2 Protein, hrvp1 Protein, rvp1 Protein, CLDN3 Protein, LOC100228090 Protein, C7orf1 Protein, CPE-R2 Protein, CPETR2 Protein, HRVP1 Protein, RVP1 Protein, AI182374 Protein, Cpetr2 Protein, mRVP1 Protein, claudin 3 L homeolog Protein, claudin 3 Protein, claudin-4-like Protein, cldn3.L Protein, cldn3 Protein, CLDN3 Protein, LOC100228090 Protein, Cldn3 Protein
- Background
- Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]
- Molecular Weight
- The human full length CLDN3 protein has a MW of 23.3 kDa
- UniProt
- O15551
- Pathways
- Hepatitis C
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