LGR4 Protein
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- Target See all LGR4 Proteins
- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human LGR4 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
- Top Product
- Discover our top product LGR4 Protein
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
- Alternative Name
- LGR4 (LGR4 Products)
- Synonyms
- BNMD17 Protein, GPR48 Protein, 9130225G07 Protein, A330106J01Rik Protein, A930009A08Rik Protein, Gpr48 Protein, leucine rich repeat containing G protein-coupled receptor 4 Protein, leucine-rich repeat-containing G protein-coupled receptor 4 Protein, LGR4 Protein, Lgr4 Protein
- Background
- Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye.
- Molecular Weight
- The human full length LGR4 protein has a MW of 104.5 kDa
- UniProt
- Q9BXB1
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