CD133 Protein
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- Target See all CD133 (PROM1) products
- CD133 (PROM1) (Prominin 1 (PROM1))
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human PROM1 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- CD133 (PROM1) (Prominin 1 (PROM1))
- Alternative Name
- PROM1 (PROM1 Products)
- Synonyms
- Prom Protein, AC133 Protein, CD133 Protein, Prom-1 Protein, Proml1 Protein, 4932416E19Rik Protein, CORD12 Protein, MCDR2 Protein, PROML1 Protein, RP41 Protein, STGD4 Protein, prominin 1 Protein, Prom1 Protein, PROM1 Protein
- Background
- A pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- The human full length PROM1 protein has a MW of 97.2 kDa
- UniProt
- O43490
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