CNR1 Protein

Catalog No. ABIN7491553

Product Details

Target: CNR1 (Cannabinoid Receptor 1 (CNR1))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purpose: Human CB1 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CNR1 (Cannabinoid Receptor 1 (CNR1))

Alternative Name: CB1 (CNR1 Products)

Synonyms: CANN6 Protein, CB-R Protein, CB1 Protein, CB1A Protein, CB1K5 Protein, CB1R Protein, CNR Protein, SKR6R Protein, cnr1-A Protein, cannabinoid receptor 1 Protein, cannabinoid receptor 1 (brain) Protein, cannabinoid receptor 1 (brain) S homeolog Protein, CNR1 Protein, Cnr1 Protein, cnr1 Protein, cnr1.S Protein

Background: The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

Molecular Weight: The human full length CB1 Protein has a MW of 52.7 kDa

UniProt: P21554

Pathways: Feeding Behaviour