MPXV E8L (AA 1-275) protein (His tag)

Catalog No. ABIN7491241

Product Details

Target: MPXV E8L

Protein Type: Recombinant

Protein Characteristics: AA 1-275

Origin: Mpox Virus

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Purpose: Recombinant Monkeypox virus E8L Protein with C-terminal 6xHis tag

Specificity: Monkeypox virus E8L (Met1-Thr275) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: MPXV E8L

Background: Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa , multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. E8L can Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.

Molecular Weight: predicted molecular mass of 32.7 kDa after removal of the signal peptide. The apparent molecular mass of Monkeypox virus E8L-His is 35-55 kDa due to glycosylation.

UniProt: Q8V4Y0