HLA-A*02:01 (Monomer) protein (WT-1)
Origin: Human, Mouse
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- HLA-A*02:01
- Protein Type
- Recombinant
- Protein Characteristics
- Monomer
- Origin
- Human, Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- WT-1
- Purpose
- Chimeric HLA-A*02:01 (mα3) & B2M & WT-1 (RMFPNAPYL) Monomer Protein
- Sequence
- Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide
- Specificity
- Uni-Prot: A0A140T913 (Human HLA-A*02:01 α1, α2), P01897 (Mouse H-2Ld α3), P61769 (B2M), RMFPNAPYL
- Characteristics
- Recombinant Chimeric HLA-A*02:01(mα3) & B2M & WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr206(Human HLA-A*02:01 α1 & α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) , His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 28.1ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HLA-A*02:01
- Background
- The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
- Molecular Weight
- 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A140T913
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