HLA-A*24:02 (AA 25-305), (Tetramer) protein (His-Avi Tag,Survivin 2B)

Catalog No. ABIN7454358

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for HLA-A*24:02 (AA 25-305), (Tetramer) protein (His-Avi Tag,Survivin 2B) (ABIN7454358)

Target: HLA-A*24:02

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-305, Tetramer

Purification tag / Conjugate: His-Avi Tag,Survivin 2B

Purpose: Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer Protein

Sequence: Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M)and AYACNTSTL peptide

Specificity: Uni-Prot: AAA59600.1 (HLA-A*24:02), P61769 (B2M), AYACNTSTL

Characteristics: Recombinant Human Survivin 2B(HLA-A*24:02) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and AYACNTSTL peptide.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLA-A*24:02

Background: Survivin-2B, a known splice variant of survivin, has been reported to promote cell death in some cancer cells, although it keeps prosurvival function in others.survivin-2B promoted autophagy and further regulated cell death by accumulating and stabilizing IKK alpha in the nucleus.

Molecular Weight: 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.