HLA-A*01:01 (Tetramer) protein (MAGE-A3)
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- HLA-A*01:01
- Protein Type
- Recombinant
- Protein Characteristics
- Tetramer
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- MAGE-A3
- Purpose
- Human HLA-A*01:01 & B2M & MAGE-A3 (EVDPIGHLY) Tetramer Protein
- Sequence
- Gly25-Thr305 (HLA-A*01:01), Ile21-Met119 (B2M) and EVDPIGHLY peptide
- Specificity
- Uni-Prot: Q5SUL5 (HLA-A*01:01), P61769 (B2M), EVDPIGHLY
- Characteristics
- Recombinant Human MAGE-A3(HLA-A*01:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*01:01),Ile21-Met119(B2M) and EVDPIGHLY peptide.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HLA-A*01:01
- Background
- Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.
- Molecular Weight
- 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
- UniProt
- Q5SUL5
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