HLA-A*03:01 (Tetramer) protein (KRAS G12V)
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- HLA-A*03:01
- Protein Type
- Recombinant
- Protein Characteristics
- Tetramer
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- KRAS G12V
- Purpose
- Human HLA-A*03:01 & B2M & KRAS G12V (VVVGAVGVGK) Tetramer Protein
- Sequence
- Gly25-Thr305 (HLA-A*03:01), Ile21-Met119 (B2M) and VVVGAVGVGK peptide
- Specificity
- Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M), VVVGAVGVGK
- Characteristics
- Recombinant Human HLA-A*03:01 & B2M & KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human KRAS G12V (HLA-A*03:01) Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-KRAS G12V (HLA-A*03:01) Antibody, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA (QC Test). The affinity constant of 42.3 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HLA-A*03:01
- Background
- Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
- Molecular Weight
- 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
- NCBI Accession
- NP_002107
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