Fibronectin Protein (AA 32-345) (His tag)

Catalog No. ABIN7454257

Product Details

Target: Fibronectin

Protein Type: Recombinant

Protein Characteristics: AA 32-345

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Fibronectin protein is labelled with His tag.

Purpose: Human Fibronectin (32-345) Protein

Sequence: Gln32-Thr345

Characteristics: Recombinant Human Fibronectin (32-345) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Thr345.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Fibronectin

Abstract: Fibronectin Products

Background: Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans.

Molecular Weight: 35.93 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.