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VEGF121 Protein (AA 27-147)

VEGF121 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Catalog No. ABIN7454231
  • Target See all VEGF121 Proteins
    VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 27-147
    Origin
    • 13
    • 1
    • 1
    Human
    Source
    • 9
    • 5
    • 1
    HEK-293 Cells
    Purpose
    Human VEGF121 Protein
    Sequence
    Ala27-Arg147
    Characteristics
    Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human VEGF121, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
    Top Product
    Discover our top product VEGF121 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
    Alternative Name
    VEGF121 (VEGF121 Products)
    Background
    Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
    Molecular Weight
    14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.
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