VEGF121 Protein (AA 27-147)
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- Target See all VEGF121 Proteins
- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-147
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human VEGF121 Protein
- Sequence
- Ala27-Arg147
- Characteristics
- Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human VEGF121, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product VEGF121 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
- Alternative Name
- VEGF121 (VEGF121 Products)
- Background
- Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
- Molecular Weight
- 14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.
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