TPBG Protein (AA 32-355) (Fc Tag)
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- Target See all TPBG Proteins
- TPBG (Trophoblast Glycoprotein (TPBG))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-355
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TPBG protein is labelled with Fc Tag.
- Purpose
- Human TPBG/5T4 Protein
- Sequence
- Ser32-Ser355
- Characteristics
- Recombinant Human TPBG/5T4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser32-Ser355.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human TPBG, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product TPBG Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TPBG (Trophoblast Glycoprotein (TPBG))
- Alternative Name
- TPBG (TPBG Products)
- Synonyms
- 5T4 Protein, 5T4AG Protein, M6P1 Protein, AW495680 Protein, TPBG Protein, tpbg Protein, trophoblast glycoprotein Protein, trophoblast glycoprotein b Protein, TPBG Protein, Tpbg Protein, tpbgb Protein, tpbg Protein
- Background
- The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
- Molecular Weight
- 62.08 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.
- UniProt
- Q13641
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