KIT Protein (AA 26-520) (His tag)

Catalog No. ABIN7454182

Product Details

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Protein Type: Recombinant

Protein Characteristics: AA 26-520

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purification tag / Conjugate: This KIT protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque CD117 Protein

Sequence: Gln26-Pro520

Characteristics: Recombinant Cynomolgus/Rhesus macaque CD117 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Pro520.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human SCF, No Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Cynomolgus CD117, His Tag with the EC50 of 4.76μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117 (KIT Products)

Synonyms: C-Kit Protein, CD117 Protein, PBT Protein, SCFR Protein, Bs Protein, Fdc Protein, Gsfsco1 Protein, Gsfsco5 Protein, Gsfsow3 Protein, SCO1 Protein, SCO5 Protein, SOW3 Protein, Ssm Protein, Tr-kit Protein, W Protein, c-KIT Protein, c-kit Protein, MGF Protein, KIT Protein, C-KIT Protein, kit Protein, receptor Protein, sparse Protein, Xkl-1 Protein, kit-a Protein, kit-b Protein, kl1-A Protein, krk1 Protein, pbt Protein, scfr Protein, KIT proto-oncogene receptor tyrosine kinase Protein, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a Protein, KIT proto-oncogene receptor tyrosine kinase S homeolog Protein, KIT Protein, Kit Protein, kita Protein, kit Protein, kit.S Protein

Background: The c-kit proto-oncogen (CD117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.

Molecular Weight: 56.69 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005555332

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity