IL-8 Protein (AA 23-100) (His tag)

Catalog No. ABIN7454155

Product Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein Type: Recombinant

Protein Characteristics: AA 23-100

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This IL-8 protein is labelled with His tag.

Purpose: Cynomolgus IL-8/CXCL8 Protein

Sequence: Ala23-Pro100

Characteristics: Recombinant Cynomolgus IL-8/CXCL8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Pro100.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus IL-8, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8 (IL8 Products)

Synonyms: CXCL8 Protein, GCP-1 Protein, GCP1 Protein, LECT Protein, LUCT Protein, LYNAP Protein, MDNCF Protein, MONAP Protein, NAF Protein, NAP-1 Protein, NAP1 Protein, IL-8 Protein, AMCF-I Protein, il8 Protein, IL8 Protein, si:dkey-151b16.2 Protein, cxcli2 Protein, cxcl8 Protein, gcp-1 Protein, il-8 Protein, lynap Protein, mdncf Protein, monap Protein, nap-1 Protein, C-X-C motif chemokine ligand 8 Protein, interleukin 8 Protein, chemokine (C-X-C motif) ligand 8a Protein, chemokine (C-X-C motif) ligand 1-like Protein, chemokine (C-X-C motif) ligand 8 L homeolog Protein, CXCL8 Protein, il8 Protein, cxcl8a Protein, IL8 Protein, Cxcl8 Protein, LOC422654 Protein, cxcl8.L Protein

Background: Interleukin-8 (IL-8) has been revealed as a critical regulator of CNS function and development with participation in many CNS disorders including gliomas.Several promising approaches that target directly or indirectly IL-8 effects in gliomas are currently in progress while more-in-depth studies are needed to validate its biomarker role and elucidate the underlying molecular mechanisms.

Molecular Weight: 10.12 kDa. Due to glycosylation, the protein migrates to 12-14 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5TUL7

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy