VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)
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- Target
- VEGF 165
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-191
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His-Avi Tag,Biotin
- Purpose
- Biotinylated Human VEGF165 Protein
- Sequence
- Ala27-Arg191
- Characteristics
- Recombinant Biotinylated Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human VEGF165 at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- VEGF 165
- Alternative Name
- VEGF165
- Background
- Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
- Molecular Weight
- 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
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