Thymic Stromal Lymphopoietin Protein (TSLP) (Arg127Ala-Mutant, Arg130Ala-Mutant) (His-Avi Tag,Biotin)
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- Target See all Thymic Stromal Lymphopoietin (TSLP) Proteins
- Thymic Stromal Lymphopoietin (TSLP)
- Protein Type
- Recombinant
- Protein Characteristics
- Arg127Ala-Mutant, Arg130Ala-Mutant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Thymic Stromal Lymphopoietin protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Tyr29-Gln159(R127A,R130A)
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product TSLP Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Thymic Stromal Lymphopoietin (TSLP)
- Alternative Name
- TSLP (TSLP Products)
- Synonyms
- TSLP Protein, thymic stromal lymphopoietin Protein, TSLP Protein, Tslp Protein
- Background
- Thymic stromal lymphopoietin, TSLP,Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
- Molecular Weight
- 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Tris-Bis PAGE result.
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