TIGIT Protein (AA 26-143) (Fc Tag)
-
- Target See all TIGIT Proteins
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-143
-
Origin
- Mouse
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TIGIT protein is labelled with Fc Tag.
- Purpose
- Mouse TIGIT Protein
- Sequence
- Gly26-Thr143
- Characteristics
- Recombinant Mouse TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly26-Thr143.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse CD155, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product TIGIT Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
- Alternative Name
- TIGIT (TIGIT Products)
- Synonyms
- VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein
- Background
- TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
- Molecular Weight
- 39.7 kDa. Due to glycosylation, the protein migrates to 48-57 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_001139797
- Pathways
- Cancer Immune Checkpoints
-