TIGIT Protein (AA 22-141) (Fc Tag)
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- Target See all TIGIT Proteins
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-141
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TIGIT protein is labelled with Fc Tag.
- Purpose
- Human TIGIT Protein
- Sequence
- Met22-Pro141
- Characteristics
- Recombinant Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met22-Pro141.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD155, mFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 73.0ng/ml determined by ELISA. The affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
- Alternative Name
- TIGIT (TIGIT Products)
- Synonyms
- VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein
- Background
- TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
- Molecular Weight
- 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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