PLAU Protein (AA 21-430) (His tag)
-
- Target See all PLAU Proteins
- PLAU (Plasminogen Activator, Urokinase (PLAU))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-430
-
Origin
- Cynomolgus
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PLAU protein is labelled with His tag.
- Purpose
- Cynomolgus PLAU/uPA Protein (active form)
- Sequence
- Ser21-Leu430
- Characteristics
- Recombinant Cynomolgus PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu430, which consists of two chains: Long chain A and chain B. The long chain A is further cleaved to yield a short chain A and N-Terminus fragment.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product PLAU Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- PLAU (Plasminogen Activator, Urokinase (PLAU))
- Alternative Name
- PLAU (PLAU Products)
- Synonyms
- PLAU Protein, u-PA Protein, ATF Protein, BDPLT5 Protein, QPD Protein, UPA Protein, URK Protein, uPA Protein, UPAM Protein, plasminogen activator, urokinase Protein, urokinase-type plasminogen activator Protein, PLAU Protein, CpipJ_CPIJ002131 Protein, CpipJ_CPIJ006543 Protein, CpipJ_CPIJ013396 Protein, CpipJ_CPIJ013623 Protein, Plau Protein
- Background
- Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
- Molecular Weight
- 47.23 kDa. Due to protein lysis and glycosylation, the protein migrates to 23-25 kDa (long chain A), 35-40 kDa (chain B) and 50-60 kDa (long chain A&chain B) based on Tris-Bis PAGE result.
- UniProt
- A0A2K5WND1
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
-