TNFRSF4 Protein (AA 29-216) (Fc Tag)
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- Target See all TNFRSF4 Proteins
- TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-216
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFRSF4 protein is labelled with Fc Tag.
- Purpose
- Human OX40/TNFRSF4/CD134 Protein
- Sequence
- Leu29-Ala216
- Characteristics
- Recombinant Human OX40 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu29-Ala216.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human OX40 Ligand (Trimer) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.27μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product TNFRSF4 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
- Alternative Name
- OX40 (TNFRSF4 Products)
- Synonyms
- TNFRSF4 Protein, ACT35 Protein, CD134 Protein, Ly-70 Protein, Ox40 Protein, TXGP1L Protein, Txgp1 Protein, OX40 Protein, TNF receptor superfamily member 4 Protein, tumor necrosis factor receptor superfamily, member 4 Protein, TNFRSF4 Protein, Tnfrsf4 Protein
- Background
- Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation, its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
- Molecular Weight
- 46.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
- UniProt
- P43489
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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