SARS-CoV-2 NSP7, NSP8 (AA 1-83) protein (His tag)
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- Target
- SARS-CoV-2 NSP7, NSP8
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-83
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Purpose
- SARS-COV-2 NSP7 & NSP8 Protein
- Sequence
- Ser1-Gln83 (NSP7) & Ala1-Gln198 (NSP8)
- Specificity
- Uni-Prot: YP_009725303.1 (NSP7), YP_009725304.1 (NSP8)
- Characteristics
- SARS-COV-2 NSP7 & NSP8 Protein is expressed from E.coil with His tag at the C-Terminus.It contains Ser1-Gln83(NSP7) & Ala1-Gln198(NSP8).
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM Nacl, 200 mM Arginine ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SARS-CoV-2 NSP7, NSP8
- Alternative Name
- SARS-CoV2 NSP7 & NSP8
- Target Type
- Viral Protein
- Background
- The crystal structure of the metabolite of remdesivir (Monophosphate of GS-441524) and NSP12-NSP8-NSP7 of SARS CoV-2 virus was recently reported. The crystal structures of ADP-Ribose or AMP and NSP3 of SARS CoV-2 virus were also released, recently. The crystal structure of NSP3 of SARS CoV-2 virus as an alternative binding site of AMP or ADP-ribose to treat COVID-19.
- Molecular Weight
- 32.8 kDa same as Tris-Bis PAGE result.
- NCBI Accession
- YP_009725303
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