NOTCH2 Protein (His-Avi Tag,Biotin)
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- Target See all NOTCH2 Proteins
- NOTCH2 (Notch 2 (NOTCH2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NOTCH2 protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Leu26-Gln530
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- NOTCH2 (Notch 2 (NOTCH2))
- Alternative Name
- Notch 2 (NOTCH2 Products)
- Synonyms
- AGS2 Protein, HJCYS Protein, hN2 Protein, AI853703 Protein, N2 Protein, notch6 Protein, sb:cb884 Protein, neurogenic locus notch homolog protein 2 Protein, notch 2 Protein, notch 3 Protein, LOC581186 Protein, NOTCH2 Protein, notch3 Protein, Notch2 Protein, notch2 Protein
- Background
- AGS2, Notch-2, NOTCH2, N2ECD, HJCYS, N2,Human Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, showing that not all functions overlap.Notch-2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
- Molecular Weight
- 57 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
- UniProt
- Q04721
- Pathways
- Notch Signaling, Stem Cell Maintenance
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