NKG2C & CD94 protein (His-Avi Tag)
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- Target
- NKG2C & CD94
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His-Avi Tag
- Sequence
- Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 1.65 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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NKG2C & CD94 (AA 98-231) protein (His tag,DYKDDDDK Tag)
Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2C & CD94 (AA 98-231) protein (His-Avi Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2C & CD94 (AA 94-231) (Active) protein (Fc Tag)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
NKG2C & CD94 (AA 94-231) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- NKG2C & CD94
- Alternative Name
- NKG2C&CD94
- Background
- NKG2C&CD94,NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.
- Molecular Weight
- 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
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