Lipocalin 2 Protein (LCN2) (AA 21-198) (His tag)
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- Target See all Lipocalin 2 (LCN2) Proteins
- Lipocalin 2 (LCN2)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-198
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Lipocalin 2 protein is labelled with His tag.
- Purpose
- Human NGAL/Lipocalin-2 Protein
- Sequence
- Gln21-Gly198
- Characteristics
- Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Gly198.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human NGAL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product LCN2 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Lipocalin 2 (LCN2)
- Alternative Name
- NGAL (LCN2 Products)
- Synonyms
- LCN2 Protein, 24p3 Protein, MSFI Protein, NGAL Protein, AW212229 Protein, Sip24 Protein, lipocalin 2 Protein, LCN2 Protein, Lcn2 Protein
- Background
- Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.
- Molecular Weight
- 21.64 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
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