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LIF Protein (AA 23-202)

LIF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7275151
  • Target See all LIF Proteins
    LIF (Leukemia Inhibitory Factor (LIF))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 23-202
    Origin
    • 16
    • 12
    • 4
    • 3
    • 2
    • 2
    • 1
    Human
    Source
    • 20
    • 13
    • 2
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purpose
    Human LIF Protein
    Sequence
    Ser23-Phe202
    Characteristics
    Recombinant Human LIF Protein is expressed from HEK293 without tag.It contains Ser23-Phe202.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human LIF at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.39μg/ml determined by ELISA. See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    LIF (Leukemia Inhibitory Factor (LIF))
    Alternative Name
    LIF (LIF Products)
    Synonyms
    CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein
    Background
    Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
    Molecular Weight
    19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
    Pathways
    JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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