TNFSF14 Protein (AA 74-240) (Fc Tag)
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- Target See all TNFSF14 Proteins
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 74-240
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFSF14 protein is labelled with Fc Tag.
- Purpose
- Human LIGHT/TNFSF14 Protein
- Sequence
- Asp74-Val240
- Characteristics
- Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp74-Val240.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product TNFSF14 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- Alternative Name
- LIGHT (TNFSF14 Products)
- Synonyms
- TNFSF14 Protein, CD258 Protein, HVEML Protein, LIGHT Protein, LTg Protein, TR2 Protein, HVEM-L Protein, Ly113 Protein, TNF superfamily member 14 Protein, tumor necrosis factor (ligand) superfamily, member 14 Protein, TNFSF14 Protein, Tnfsf14 Protein
- Background
- LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
- Molecular Weight
- 45.5 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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