ITGAV/ITGB8 Protein (AA 31-993) (His tag)
-
- Target See all ITGAV/ITGB8 products
- ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-993
-
Origin
- Rhesus Monkey
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ITGAV/ITGB8 protein is labelled with His tag.
- Purpose
- Rhesus macaque Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer Protein
- Sequence
- Phe31-Pro993 (ITGAV) acidic tail & Glu43-Ser681 (ITGB8) basic tail
- Specificity
- Uni-Prot: F6QSK2 (ITGAV), F7F3K9 (ITGB8)
- Characteristics
- Recombinant Rhesus macaque Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe31-Pro993(ITGAV) acidic tail & Glu43-Ser681(ITGB8) basic tail.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Rhesus macaque ITGAV&ITGB8, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha-v Antibody , hFc Tag with the EC50 of 56ng/ml determined by ELISA. See testing image for detail.
-
Integrin alpha V beta 8 (ITGAV/ITGB8) (AA 31-988) (Active) protein (His tag)
ITGAV/ITGB8 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Integrin alpha V beta 8 (ITGAV/ITGB8) (AA 31-992) (Active) protein (His tag)ITGAV/ITGB8 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Integrin alpha V beta 8 (ITGAV/ITGB8) (AA 31-992) protein (His tag,AVI tag,Biotin)ITGAV/ITGB8 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))
- Alternative Name
- Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer (ITGAV/ITGB8 Products)
- Background
- Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation.
- Molecular Weight
- 112.1 kDa (ITGAV)&75.3 kDa (ITGB8). Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result.
- UniProt
- F6QSK2
-
