CD51/CD61 Protein (AA 31-992) (His-Avi Tag)
-
- Target See all CD51/CD61 (ITGAV/ITGB3) Proteins
- CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-992
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD51/CD61 protein is labelled with His-Avi Tag.
- Purpose
- Human Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein
- Sequence
- Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail
- Specificity
- Uni-Prot: P06756-1 (ITGAV), P05106-1 (ITGB3)
- Characteristics
- Recombinant Human Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992(ITGAV) acidic tail & GLy27-Asp718(ITGB3) basic tail.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ITGAV&ITGB3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))
- Alternative Name
- Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer (ITGAV/ITGB3 Products)
- Synonyms
- CD51 Protein, MSK8 Protein, VNRA Protein, VTNR Protein, integrin subunit alpha V Protein, ITGAV Protein
- Background
- ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.
- Molecular Weight
- 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation,the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.
-