IL23R Protein (AA 24-355) (His tag)
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- Target See all IL23R Proteins
- IL23R (Interleukin 23 Receptor (IL23R))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-355
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL23R protein is labelled with His tag.
- Purpose
- Human IL-23R Protein
- Sequence
- Gly24-Gly355
- Characteristics
- Recombinant Human IL-23R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly355.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-23R, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 1.15μg/ml determined by ELISA. The affinity constant of 5.72 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL23R (Interleukin 23 Receptor (IL23R))
- Alternative Name
- IL-23R (IL23R Products)
- Synonyms
- IL-23R Protein, IL23R Protein, il-23r Protein, interleukin 23 receptor Protein, IL23R Protein, il23r Protein, Il23r Protein
- Background
- Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R).This receptor associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade.
- Molecular Weight
- 39.15 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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