IL-15 Protein
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- Target See all IL-15 (IL15) Proteins
- IL-15 (IL15) (Interleukin 15 (IL15))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- Asn49-Ser162
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-15 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 12.1ng/ml determined by ELISA. The affinity constant of 0.056 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM NaCl ( pH 8.0). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-15 (IL15) (Interleukin 15 (IL15))
- Alternative Name
- IL-15 (IL15 Products)
- Synonyms
- IL15 Protein, AI503618 Protein, IL-15 Protein, interleukin 15 Protein, IL15 Protein, Il15 Protein
- Background
- L15, IL-15, IL-15MGC9721, Interleukin-15,Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases.IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
- Molecular Weight
- 12.8 kDa same as Tris-Bis PAGE result.
- UniProt
- P40933
- Pathways
- JAK-STAT Signaling, Glycosaminoglycan Metabolic Process
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