IL-21 Protein
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- Target See all IL-21 (IL21) Proteins
- IL-21 (IL21) (Interleukin 21 (IL21))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- Gln32-Ser162
- Purity
- > 95% as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-21, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 63.6ng/ml determined by ELISA. See testing image for detail.
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Interleukin 21 (IL21) (AA 30-162) protein (Fc Tag)
IL21 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Interleukin 21 (IL21) (AA 30-162) (Active) proteinIL21 Origin: Human Host: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Interleukin 21 (IL21) (AA 32-162) protein (His-Avi Tag,Biotin)IL21 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 21 (IL21) (AA 18-146) protein (His-IF2DI Tag)IL21 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 21 (IL21) (AA 25-155) protein (His-IF2DI Tag)IL21 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 21 (IL21) (AA 30-162) protein (His tag)IL21 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 21 (IL21) (AA 30-162) protein (mFc Tag)IL21 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 21 (IL21) (AA 1-162) protein (GST tag)IL21 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 21 (IL21) (AA 63-162) protein (GST tag)IL21 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 21 (IL21) (AA 18-146) protein (His-Avi Tag,Biotin)IL21 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.5).
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.5). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-21 (IL21) (Interleukin 21 (IL21))
- Alternative Name
- IL-21 (IL21 Products)
- Synonyms
- IL-21 Protein, Za11 Protein, interleukin 21 Protein, IL21 Protein, Il21 Protein
- Background
- Interleukin-21, IL-21, Za11, CVID11,Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
- Molecular Weight
- 15.4 kDa same as Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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