IL12 Protein (His-Avi Tag)
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- Target See all IL12 Proteins
- IL12 (Interleukin 12 (IL12))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL12 protein is labelled with His-Avi Tag.
- Sequence
- Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A)
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
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Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag,Biotin)
IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) proteinIL12 Origin: Human Host: HEK-293T Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 12 (IL12) (AA 23-215) protein (His tag)IL12 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,DYKDDDDK Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-219) protein (Fc-His Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 12 (IL12) proteinIL12 Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 12 (IL12) proteinIL12 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 12 (IL12) protein (His tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL12 (Interleukin 12 (IL12))
- Alternative Name
- IL-12 (IL12 Products)
- Background
- CLMF, CLMF2, IL-12A, IL-12B, IL12, IL12 p70, IMD28, IMD29, NFSK, NKSF1, NKSF2, P35, IL-12 subunit p35, IL12A, interleukin 12,Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
- Molecular Weight
- 34.7(IL-12B)/25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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