IL-13 Protein (AA 21-132) (Fc Tag)
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- Target See all IL-13 (IL13) Proteins
- IL-13 (IL13) (Interleukin 13 (IL13))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-132
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL-13 protein is labelled with Fc Tag.
- Purpose
- Human IL-13 Protein
- Sequence
- Gly21-Asn132
- Characteristics
- Recombinant Human IL-13 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gly21-Asn132.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-13, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 2.8μg/ml determined by ELISA. The affinity constant of 0.399 μM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product IL13 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-13 (IL13) (Interleukin 13 (IL13))
- Alternative Name
- IL-13 (IL13 Products)
- Synonyms
- IL13 Protein, IL-13 Protein, P600 Protein, Il-13 Protein, L13 Protein, interleukin 13 Protein, IL13 Protein, Il13 Protein
- Background
- Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
- Molecular Weight
- 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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