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IL-18 Protein

IL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Catalog No. ABIN7274880
  • Target See all IL-18 (IL18) Proteins
    IL-18 (IL18) (Interleukin 18 (IL18))
    Protein Type
    Recombinant
    Origin
    • 17
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 26
    • 9
    • 3
    • 2
    • 1
    Escherichia coli (E. coli)
    Sequence
    Tyr37-Asp193
    Purity
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. The affinity constant of 1.21nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
    Storage
    4 °C,-80 °C
    Storage Comment
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    IL-18 (IL18) (Interleukin 18 (IL18))
    Alternative Name
    IL-18 (IL18 Products)
    Synonyms
    Igif Protein, Il-18 Protein, IL-18 Protein, IGIF Protein, ChIL-18 Protein, IL-1g Protein, IL1F4 Protein, IL18 Protein, interleukin 18 Protein, Il18 Protein, IL18 Protein
    Background
    IL-18, IL18, IGIF, IL-1F4, IL-1 gamma, IL1F4, IL-1g, Interleukin 1,Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
    Molecular Weight
    18.2 kDa same as Tris-Bis PAGE result.
    UniProt
    Q14116
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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