IgE Protein (AA 198-421) (His-Avi Tag)
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- Target See all IgE Proteins
- IgE
- Protein Type
- Recombinant
- Protein Characteristics
- AA 198-421
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IgE protein is labelled with His-Avi Tag.
- Purpose
- Mouse IgE Protein
- Sequence
- Asp198-Ser421
- Characteristics
- Recombinant Mouse IgE Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp198-Ser421.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product IgE Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IgE
- Abstract
- IgE Products
- Synonyms
- IGH2 Protein, IgE Protein, Gm900 Protein, immunoglobulin heavy chain (epsilon polypeptide) Protein, immunoglobulin heavy constant epsilon Protein, Immunoglobulin heavy constant epsilon Protein, Ighe Protein, IGHE Protein
- Background
- Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.
- Molecular Weight
- 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
- UniProt
- P06336
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