IFNA Protein (His-Avi Tag)

Catalog No. ABIN7274849

Product Details

Target: IFNA (Interferon alpha (IFNA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IFNA protein is labelled with His-Avi Tag.

Sequence: Lys28-Lys436

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IFNAR1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 0.12 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IFNA (Interferon alpha (IFNA))

Alternative Name: IFN alpha (IFNA Products)

Synonyms: IFN-alphaO Protein, IFN-ALPHA-1 Protein, IFN1@ Protein, Ifa Protein, Ifa8 Protein, interferon alpha 16 Protein, interferon, alpha 1 Protein, interferon alpha Protein, interferon-alpha Protein, IFNA16 Protein, IFNA1 Protein, Ifna Protein, IFNA Protein

Background: AVP, IFN-alpha/beta R1, IFN-alpha-REC, IFNAR, IFNAR1, IFN-aR1, IFNBR, IFNbR1, IFN-R-1, CRF2-1, IFRC,IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.

Molecular Weight: 50.2 kDa. Due to glycosylation, the protein migrates to 74-95 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome